Table of Contents18.417 Lecture 13 Announcements Continuing our journey of the central dogma Gene Regulation Why the cell needs to change Fixed genome in a changing cell Regulatory knobs at every step Why study transcription initiation Sequence-dependent regulation Transcription Initiation Machinery Regulatory sequence signals Steps to transcription Eukaryotic packaging Observing Regulation The idea behind DNA MicroArrays The first RNA expression observation Today Two ways of making DNA Arrays Printed arrays: fixing DNA onto glass DNA chips: Growing oligos on glass Fluorescently labeling the samples Printed Array: Comparing two conditions DNA chips: measuring absolute expression DNA chips: measuring absolute expression Modeling noise Next time: Regulatory Networks |
Author: Manolis
Email: manoli@mit.edu Home Page: http://theory.lcs.mit.edu/~bab/01-18.417-home.html Other information: |