18.417 Lecture 9

Introduction

Before the Revolution

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Recombinant DNA operations

Cutting

Pasting

Copying

Amplifying with flanking primers

PCR: polymerase chain reaction

Making the primers

Why are all those proteins needed?

Sequencing Reaction

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Learning where the Ts are

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From dyes to sequence

Sequencing a small genome

Sequencing a small genome

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From plates to sequence

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Production Details

Finishing

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Sequencing a Large Genome

Two levels of libraries

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Next time: AssemblyNow: Let’s look at the lab

Further Reading